Optimisation of PCR reactions using primer chessboarding.
نویسندگان
چکیده
In-house polymerase chain reaction (PCR) assays are now an integral part of the work of most diagnostic microbiological laboratories. Despite the availability of commercial reagent 'master-mixes' of some PCR reagents, the optimisation of primers still poses a significant problem. Here, we describe a simple method to assess the concentration of primer needed in single round, multiplex, nested and 'real-time' PCR procedures.
منابع مشابه
Performing Real-Time PCR
Optimisation of the reagents used to perform PCR is critical for reliable and reproducible results. As with any PCR initial time spent on optimisation of a real-time assay will be benefi cial in the long run. Specifi city, sensitivity, effi ciency and reproducibility are the important criteria to consider when optimising an assay and these can be altered by changes in the primer concentration, ...
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ورودعنوان ژورنال:
- Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
دوره 26 3 شماره
صفحات -
تاریخ انتشار 2003